Anti-CD14 Antibody, FITC-Labeled

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Catalog #

101884

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Description

This anti-CD14 antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for binding to human CD14 on the surface of CD14 CHO Recombinant Cell Line (BPS Bioscience #78335).

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Synonyms

Monocyte differentiation antigen CD14, CD_antigen CD14

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Product Data Gallery

Cell surface expression of CD14 in CD14 CHO Cell Line.

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatant.

Format

Aqueous buffer solution.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

UniProt #

P08571

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Background

CD14 (Cluster of Differentiation-14) is a surface antigen expressed predominantly by monocytes and macrophages. It is considered a patterned recognition receptor (PRR), which recognizes pathogen markers. Specifically, CD14 acts as a co-receptor for lipopolysaccharides (LPS) found in the outer membrane of gram-negative bacteria. Through this interaction, CD14 supports initiation of the innate immune response during bacterial infection. CD14 also contributes toward immune responses to viral pathogens such as human respiratory syncytial virus (RSV) and may amplify the inflammatory response observed in severe cases of SARS-CoV-2 infection (COVID-19). Monoclonal antibodies against CD14 are currently undergoing clinical trial as a candidate treatment to limit severe inflammatory responses common in patients hospitalized with COVID-19.

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million CD14 CHO cells (BPS Bioscience #78335) were blocked and stained with 1 µg of anti-CD14 antibody, FITC-labeled (BPS Bioscience #101884) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD14 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis represents the cell count, while X-axis shows PE intensity.

Applications

Useful for studying expression of CD14 on the cell surface by flow cytometry. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human CD14. It has not been tested on other species.

Warnings

Do not freeze. Protect from light.

Scientific Category

Cell Therapy

Regulatory Status

Research Use Only

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Anti-CD14 Antibody, FITC-Labeled

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