CD14 Positive Cell Isolation Kit
The CD14 Positive Cell Isolation Kit is designed to magnetically separate CD14-expressing cells from a complex immune cell population. This kit is optimized for the isolation of CD14 positive cells from human peripheral blood mononuclear cells (PBMCs). Cells are incubated with an antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, your CD14 positive cells will be immobilized along the side of the tube while undesired CD14-negative cells will remain in suspension and can be easily removed.
- Peripheral blood mononuclear cells (PBMCs) (BPS Bioscience #79059)
- Cell Isolation Magnetic Tube Rack (BPS Bioscience #78571)
- Centrifuge
- 15- or 50-ml tubes
- Cell counter
1 x 108 Cells
| Catalog # | Name | Amount | Storage |
| Cell Isolation Magnetic Beads | 200 µl | +4°C | |
| CD14 Cell Isolation Antibody | 40 µl | -20°C | |
| 78563 | 5x Cell Isolation Buffer | 25 ml | +4°C |
1 x 109 Cells
| Catalog # | Name | Amount | Storage |
| Cell Isolation Magnetic Beads | 2 ml | +4°C | |
| CD14 Cell Isolation Antibody | 400 µl | -20°C | |
| 78563 | 5x Cell Isolation Buffer | 250 ml | +4°C |
Monocytes are cytokine-producing and phagocytic innate immune cells. These cells are of interest in the study of autoimmune diseases, cancer immunotherapy, inflammatory disorders, and other fields. CD14 is a marker of cells from the myelomonocyte lineage, including monocytes and macrophages. In PBMCs derived from healthy individuals, about 10-20% of the cells are CD14+ cells.
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