CD14 CHO Recombinant Cell Line

Catalog #

78335

$7,150 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant clonal CHO stable cell line constitutively expressing full length human CD14 protein. The surface expression of CD14 in this cell line was validated by flow cytometry.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Monocyte differentiation antigen CD14, CD_antigen CD14

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Product Data Gallery

Confirmation of CD14 expression on CHO-K1 recombinant cells

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3D	BPS Bioscience #79539

UniProt #

P08571

Mycoplasma Testing

The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.

Background

CD14 (Cluster of Differentiation-14) is a surface antigen expressed predominantly by monocytes & macrophages. It is considered a patterned recognition receptor (PRR), which recognizes pathogen markers. Specifically, CD14 acts as a co-receptor for lipopolysaccharides (LPS) found in the outer membrane of gram-negative bacteria. Through this interaction, CD14 supports initiation of the innate immune response during bacterial infection. CD14 has also been found to contribute toward immune responses to viral pathogens such as human respiratory syncytial virus (RSV), and may amplify the inflammatory response observed in severe cases of SARS-CoV-2 infection (COVID-19). Monoclonal antibodies against CD14 are currently undergoing clinical trial as a candidate treatment to limit severe inflammatory responses common in patients hospitalized with COVID-19

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3D (BPS Bioscience #79539): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml Geneticin

Instructions for Use

See assay protocol for detailed instructions

Applications

1. Useful for screening or performing binding assays of antibodies designed against human CD14 in a cellular context.
2. Cell-based assays for the assessment of CD14-mediated cell signaling and/or genetic responses under various experimental conditions (eg. inhibition/neutralization or activation/amplification).

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only