CD38 / CD19 / Firefly Luciferase CHO Recombinant Cell Line

Catalog #

78149

$10,340 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant clonal stable CHO cell line constitutively expressing full length human CD38 protein (also known as ADPRC1, Genbank accession #NM_001775) and human CD19 protein (also known as B4 or CVID3, Genbank accession #NM_001770). This cell line was derived from our CHO-K1 Luciferase cells (BPS Bioscience, #79725), therefore it also constitutively expresses the firefly luciferase reporter. Surface expression of CD38 and CD19 were confirmed by flow cytometry.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

cluster of differentiation 38 cells, ADP-Ribosyl Cyclase 1 cell line, NAD(+) Nucleosidase cell line, ADPRC 1 cell line, TNFRSF17 cells, Cluster of Differentiation 19 cell lines, B-lymphocyte surface antigen B4 cells

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Product Data Gallery

Expression of CD38 validated by flow cytometry

Expression of CD19 validated by flow cytometry

Luciferase activity of CD38 / CD19 / Firefly Luciferase - CHO Recombinant Cells

Product Info

Storage and Usage

Citations

Host Cell Line

CHO K1 cell line, Chinese Hamster Ovary, epithelial-like cells, adherent

Species

Human

Supplied As

2 vials of frozen cells. 2 x 10⁶ cells in 1 ml of 10% DMSO

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3K	BPS Bioscience #78041

Materials Required for Cellular Assay

Name	Ordering Information
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well tissue culture-treated white clear-bottom assay plate
Luminometer

UniProt #

P28907, P15391

Mycoplasma Testing

The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.

Background

The CD38 protein is a dimeric, non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes the second messengers cyclic ADP-ribose and NADP. CD38 is highly expressed by lymphoid and myeloid cells, particularly plasma cells. Increased CD38 expression on chronic lymphocytic leukemia (CLL) cells is linked to aggressive disease features and poor clinical outcome. CD38 is used as a prognostic marker for patients with CLL and multiple myeloma (MM), and is an ideal target for immunotherapy in CLL and MM.

B-lymphocyte antigen CD19 (Cluster of Differentiation 19), also known as B-Lymphocyte Surface Antigen B4 and CVID3, is a transmembrane protein expressed in follicular dendritic cells and all B lineage cells except plasma cells. CD19 plays two major roles in human B cells. It acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane and it works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. Due to its presence on all B cells, it is a biomarker for B lymphocyte development and lymphoma diagnosis and can be used as a target for leukemia immunotherapies. CD19-targeted therapies based on T cells that express CD19-specific chimeric antigen receptors (CARs) have been utilized for their antitumor abilities in patients with CD19+ lymphoma and leukemia, such as Non-Hodgkin’s Lymphoma (NHL), CLL and ALL.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience, #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3K (BPS Bioscience #78041): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1000 μg/ml Geneticin, 5 μg/ml Puromycin and 500 μg/ml of Hygromycin B to ensure recombinant expression.

Assay Medium: Thaw Medium 3 (BPS Bioscience, #60186)

Instructions for Use

See assay protocol for detailed instructions.

Applications

1. Useful for validation of anti-CD38 and anti-CD19 bispecific antibody.
2. Useful as CD38- and/or CD19-expressing target cells in co-culture assay with CD38- and/or CD19-CAR-T cells, for both CD38/CD19-specific cell killing assay and cytokine production assay.
3. Useful for screening and validating antibodies against CD38 or CD19 and anti CD38 or anti-CD19 CAR-T for immunotherapy research and drug discovery.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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CD38 / CD19 / Firefly Luciferase CHO Recombinant Cell Line

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