Firefly Luciferase NALM6 Cell Line

Catalog #

78494

$1,950 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Firefly Luciferase NALM6 Cell Line is a NALM6 cell line constitutively expressing the firefly (Photinus pyralis) luciferase reporter gene under the control of a CMV promoter.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity in Firefly Luciferase NALM6 Cell Line

Product Info

Storage and Usage

Citations

Host Cell Line

NALM6, human precursor B cell lymphoblasts derived from a patient with acute lymphoblastic leukemia, suspension

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2D	BPS Bioscience #79639

Materials Required for Cellular Assay

Name	Ordering Information
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well Flat Clear Bottom White Polystyrene TC-treated Microplates	Corning #3610
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

NALM6 is a human B cell precursor leukemia cell line derived from the peripheral blood of a patient with acute lymphoblastic leukemia. NALM6 is a unique cell line that contains features that allow for highly efficient gene targeting via homologous recombination, the process of random gene shuffling, or nucleotide cross-over. The cell line also contains a near-diploid karyotype and is easy to transfect, making NALM6 an ideal model for knock-out or knock-in studies of gene functions. The signal generated by the firefly luciferase is proportional to cell numbers.

References

Li Y, Zuo C, Gu L., 2021Cancer Cell Int. 21(1):623.
Adachi N, Nishijima H, Shibahara K., 2008 Biosci Trends. 2(5):169-180.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2D (BPS Bioscience #79639): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 μg/ml of Hygromycin B.

Applications

Use as an internal control in CAR-T or NK co-culture killing assays
In vitro and in vivo bioluminescence imaging

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only