Anti-CD19 IgG Antibody

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Catalog #

100981

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Description

This purified recombinant monoclonal antibody is a human Anti-CD19 IgG which recognizes CD19 antigen. The variable regions for this antibody are based on an FDA-approved drug. This antibody has been tested for specific binding affinity to purified human CD19 protein (BPS Bioscience, #101015) in a colorimetric ELISA binding assay.

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Synonyms

B4 antibody, CVID3 antibody, B-lymphocyte antigen CD19, T-cell surface antigen Leu-12

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Binding affinity of Anti-CD19 IgG to target protein in ELISA assay

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Host Species/Expression System

Heavy chain (HC) and Light chain (LC) co-expressed in HEK293 cells

Purity

≥90%

Supplied As

Aqueous buffer solution.

Purification

Protein A affinity purification from HEK293 cells.

Formulation

8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

HC: ~49.9 kDa; LC: ~23.9 kDa

UniProt #

P15391

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

Purified human his-tagged CD19 was bound to clear 96-well nickel plate overnight in 4°C (1 µg/mL). Upon washing and blocking wells for 1 hour at room temp, serial dilutions of anti-CD19 (200 nM to 0nM in 3-fold dilutions) were incubated in each well for 1 hr at room temp (slow shaking). Next, wells were washed and incubated with anti-human IgG-HRP for 1 hour at room temp (slow shaking) then washed again. For detection, the wells were incubated with Colorimetric HRP Substrate (BPS Bioscience #79651) for 1-10 minutes until a blue color developed in the positive control. The reaction was then quenched with equal volume of 1N HCL and absorbance read at 450 nm.

Applications

The anti-CD19 IgG format antibody can be used for labeling cells expressing CD19 for flow cytometry and immunofluorescence microcopy.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

100981 Lot 210224-1

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Anti-CD19 IgG Antibody

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