Firefly Luciferase CCRF-CEM Cell Line

Catalog #

78495

$1,900 *

Size: 2 vials

Qty

Bulk/Custom

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Description

Recombinant CCRF-CEM cells constitutively expressing the firefly (Photinus pyralis) luciferase reporter gene under the control of a CMV promoter.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity in Firefly Luciferase CCRF-CEM recombinant cells

Product Info

Storage and Usage

Citations

Host Cell Line

CCRF-CEM, human T lymphoblasts derived from a patient with acute lymphoblastic leukemia, suspension

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2D	BPS Bioscience #79639

Materials Required for Cellular Assay

Name	Ordering Information
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well Flat Clear Bottom White Polystyrene TC-treated Microplates	Corning #3610
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

CCRF-CEM is a human leukemia cell line derived from the peripheral blood of a patient with acute lymphoblastic leukemia. CCRF-CEM contains a somatic mutation that impairs the glucocorticoid receptor, which has been linked to acute lymphoblastic leukemia. The CCRF-CEM cell line provides a valuable model for studying possible roles that glucocorticoids play in leukemic cell growth. The signal generated by the firefly luciferase is proportional to cell numbers.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2D (BPS Bioscience #79639): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 μg/ml of Hygromycin B.

Applications

Use as an internal control in CAR-T or NK co-culture killing assays
In vitro and in vivo bioluminescence imaging

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only