Anti-CD8 Antibody, PE-Labeled

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Catalog #

102011

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Description

This anti-CD8 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for specific binding to human CD8 on the surface of PBMCs (BPS Bioscience #79059).

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Product Data Gallery

Cell surface expression of CD8 in PBMCs.

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatants.

Format

Aqueous buffer solution.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

Label

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa).

Background

CD8 is a co-receptor for the TCR (T cell receptor) in T cells, binding to MHC (major histocompatibility complex) class I proteins. CD8 is a typical marker of cytotoxic T cells and is involved in signaling. CD8 has two isoforms, a and b. CD8b recruits Lck (lymphocyte-specific protein tyrosine kinase) to the TCR-CD3 complex, and Lck phosphorylates multiple proteins involved in activation of cytotoxic T lymphocytes. It is thus critical for the lysis of cancer cells.

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million PBMC cells (BPS Bioscience #79059) were blocked and stained with 1 µg of anti-CD8 antibody, PE-labeled (BPS Bioscience #102011) or PE Human IgG1 Isotype Control (BioLegend #403503) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. Non-lymphoid cells were excluded from the analysis based on side scatter and forward scatter signals. Y-axis represents the cell count while X-axis indicates PE intensity.

Instructions for Use

For flow cytometry staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Applications

Useful for studying expression of CD8 on the cell surface by flow cytometry.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human CD8. It has not been tested on other species.

Warnings

Do not freeze. Protect from light.

Scientific Category

Immune Cell Markers

Regulatory Status

Research Use Only

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Anti-CD8 Antibody, PE-Labeled

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