CD8a/CD8b Jurkat Cell Line

Catalog #

78917

$3,950 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD8a/CD8b Jurkat Cell Line are human T lymphocyte Jurkat cells (Clone# E6-1) engineered to express CD8a (NM_ 001768.6) and CD8b (NM_004931.5) driven by an EF1a promoter. The cells were generated by transduction with CD8a/CD8b Lentivirus (BPS Bioscience #78650), which is a SIN (self-inactivating) lentivirus. The cells also express very low levels of endogenous CD4.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

CD8a and CD8b expression in the CD8aCD8b Jurkat Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat (clone# E6-1), human T lymphocyte cell line, suspension.

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2E	BPS Bioscience #79638

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

CD8 (cluster of differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that functions within the immune system to mediate cell-cell interactions. CD8 is a co-receptor for the TCR (T cell receptor) in T cells, binding to MHC (major histocompatibility complex) class I proteins. CD8 is a typical marker of cytotoxic T cells and is involved in signaling. CD8 has two isoforms, a and b. CD8b recruits Lck (lymphocyte-specific protein tyrosine kinase) to the TCR-CD3 complex, and Lck phosphorylates multiple proteins involved in activation of cytotoxic T lymphocytes. It is thus critical for the lysis of cancer cells. Studying CD8 and its importance in iso-immunity can further our understanding of post-transplant recognition and its potential for cancer therapy.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2E (BPS Bioscience #79638): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.5 µg/ml of Puromycin

Applications

Study CD8/MHCI complex formation and inhibition.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immune Cell Signaling

Regulatory Status

Research Use Only