Anti-CD22 CAR Lentivirus (Clone m971 ScFv-CD8-4-1BB-CD3ζ)
The anti-CD22 CAR lentiviruses are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv (single-chain variable fragment) of anti-CD22 (clone m971) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (below).
Schematic of the lenti-vector used to generate the anti-CD22 CAR lentivirus
Construct diagram showing components of the anti-CD22 CAR
Name | Ordering Information |
PBMC, Frozen | BPS Bioscience #79059 |
Human Interleukin-2 | BPS Bioscience #90184 |
EasySep™ Human CD4+ T Cell Isolation Kit | Stemcell technologies #17952 |
EasySep™ Human CD8+ T Cell Isolation Kit | Stemcell technologies #17953 |
Human CD3/CD28/CD2 T Cell Activator | Stemcell technologies #10970 |
Biotinylated Human CD22 Protein | Acrobiosystems #SI2-H82F8-25ug |
PE-Streptavidin | Biolegend #405203 |
IFN-γ (Human) Colorimetric ELISA Detection Kit | BPS Bioscience #79777 |
CD22/Firefly Luciferase CHO Cell Line | BPS Bioscience #79715 |
Firefly Luciferase CHO Cell Line | BPS Bioscience #79725 |
Firefly Luciferase Raji Cell Line | BPS Bioscience #78622 |
Firefly Luciferase K562 Cell Line | BPS Bioscience #78621 |
ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM.
Recommended CD4+CD8+ T Cell Medium: StemSpan SFEM (Stemcell Technologies, #09650) supplemented with 10% heat-inactivated FBS (Life Technologies, #10082147), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01), plus 10 ng/ml IL-2 (BPS Bioscience, #90184)