IFN-γ (Human) Colorimetric ELISA Detection Kit

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Catalog #

79777

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Description

IFN-γ is a cytokine that is primarily secreted by activated T cells and natural killer (NK) cells and plays an important role in activating innate/adaptive immunity. It also has been recognized as an indicator of T cell activation suggesting IFN-γ activation may be a prerequisite for CAR-T cell activity. The IFN-γ Colorimetric ELISA Detection Kit is designed for detecting and quantifying human interferon-γ in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IFN-γ are incubated on the coated plate followed by detecting the captured IFN-γ with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

This product has been cited 3 times.

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Synonyms

IFNG, IFG, IFI, interferon, interferon gamma

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Product Data Gallery

Detection of the human IFN-γ (left:2 pg/ml –500 pg/ml, right: 2 pg/ml – 15 pg/ml) standard using the Colorimetric Human IFN-γ Detection Kit

IFN-γ production of anti-CD20 CAR-T cells

IFN-γ production of anti-Mesothelin CAR-T cells

Product Info

Storage and Usage

Citations3

Assay Kit Format

Colorimetric

Supplied As

This kit comes in a convenient 96-well format, with capture and detection antibodies for IFN-γ, streptavidin-labeled HRP, blocking buffer, IFN-γ standard, and colorimetric HRP substrate for a 96-well plate

Materials Required But Not Supplied

PBS (Phosphate-buffered saline)
PBST (PBS with 0.05% Tween-20)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used without adding 1N HCl, but sensitivity of the assay will be reduced.

Format

96 Reactions:

Catalog #	Component	Amount	Storage
	Interferon-γ capture antibody	10 µl	-80°C	Avoid freeze/ thaw cycles!
	Interferon-γ detection antibody, biotinylated	5 µl	-80°C
	Human IFN-γ standard (1 µg/ml)	20 µl	-80°C
80611	Streptavidin-HRP	5 µl	+4°C
79743	Blocking Buffer 3	50 ml	+4°C
79651	Colorimetric HRP substrate	10 ml	+4°C
79964	Transparent 96-well microplate	1	+4°C

5 x 96 Reactions:

Catalog #	Component	Amount	Storage
	Interferon-γ capture antibody	5 x 10 µl	-80°C	Avoid freeze/ thaw cycles!
	Interferon-γ detection antibody, biotinylated	5 x 5 µl	-80°C
	Human IFN-γ standard (1 µg/ml)	5 x 20 µl	-80°C
80611	Streptavidin-HRP	5 x 5 µl	+4°C
79743	Blocking Buffer 3	5 x 50 ml	+4°C
79651	Colorimetric HRP substrate	5 x 10 ml	+4°C
79964	Transparent 96-well microplate	5 x 1	+4°C

UniProt #

P01579

References

Billiau, A., 1996. Interferon gamma: biology and role in pathogenesis, Adv. Immunology, 62:61-130

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions. All samples and standards should be tested in duplicate.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cytokine

Regulatory Status

Research Use Only