IL-2 (Human) Colorimetric ELISA Detection Kit

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Catalog #
79774
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Description

The cytokine protein Interleukin-2 is an important factor for the maintenance of CD4+ regulatory T cells. It plays a critical role in the differentiation of CD4+ T cells into a variety of subsets as well as promotes CD8+ T-cell and NK cell cytotoxicity. The IL-2 (Human) Colorimetric Detection Kit is designed for detecting and quantifying human interleukin-2 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IL-2 are incubated on the coated plate followed by detecting the captured IL-2 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

Product Info
Storage and Usage
Citations
Assay Kit Format
Colorimetric
Species
Human
Supplied As
This kit comes in a convenient 96-well format, with capture and detection antibodies for IL-2, streptavidin-labeled HRP, blocking buffer, IL-2 standard, and colorimetric HRP substrate for a 96- well plate.
Format

96 Reactions: 

CatalogNumber

Component

Amount

Storage

 

Interleukin-2 capture antibody

20 µl

-80°C

(Avoid freeze/ thaw cycles!)

 

Interleukin-2 detection antibody, biotinylated

5 µl

-80°C

 

Human IL-2 standard (1 µg/ml)

20 µl

-80°C

80611

Streptavidin-HRP

5 µl

+4°C

79743

Blocking Buffer 3

50 ml

+4°C

79651

Colorimetric HRP substrate

10 ml

+4°C

 

Transparent 96-well microplate 

1

+4°C

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

5 x 96 Reactions:

CatalogNumber

Component

Amount

Storage

 

Interleukin-2 capture antibody

5 x 20 µl

-80°C

(Avoid freeze/ thaw cycles!)

 

Interleukin-2 detection antibody, biotinylated

5 x 5 µl

-80°C

 

Human IL-2 standard (1 µg/ml)

40 µl

-80°C

80611

Streptavidin-HRP

5 x 5 µl

+4°C

79743

Blocking Buffer 3

5 x 50 ml

+4°C

79651

Colorimetric HRP substrate

5 x 10 ml

+4°C

 

Transparent 96-well microplate 

5 x 1

+4°C

UniProt #
P60568
References

Jiang, T., et al. Role of IL-2 in Cancer Immunotherapy. OncoImmunology, 2016. 5(6): e1163462