IL-2 (Human) Colorimetric ELISA Detection Kit
The cytokine protein Interleukin-2 is an important factor for the maintenance of CD4+ regulatory T cells. It plays a critical role in the differentiation of CD4+ T cells into a variety of subsets as well as promotes CD8+ T-cell and NK cell cytotoxicity. The IL-2 (Human) Colorimetric Detection Kit is designed for detecting and quantifying human interleukin-2 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IL-2 are incubated on the coated plate followed by detecting the captured IL-2 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
96 Reactions:
CatalogNumber |
Component |
Amount |
Storage |
|
|
Interleukin-2 capture antibody |
20 µl |
-80°C |
(Avoid freeze/ thaw cycles!) |
|
Interleukin-2 detection antibody, biotinylated |
5 µl |
-80°C |
|
|
Human IL-2 standard (1 µg/ml) |
20 µl |
-80°C |
|
5 µl |
+4°C |
|||
50 ml |
+4°C |
|||
79651 |
Colorimetric HRP substrate |
10 ml |
+4°C |
|
|
Transparent 96-well microplate |
1 |
+4°C |
5 x 96 Reactions:
CatalogNumber |
Component |
Amount |
Storage |
|
|
Interleukin-2 capture antibody |
5 x 20 µl |
-80°C |
(Avoid freeze/ thaw cycles!) |
|
Interleukin-2 detection antibody, biotinylated |
5 x 5 µl |
-80°C |
|
|
Human IL-2 standard (1 µg/ml) |
40 µl |
-80°C |
|
5 x 5 µl |
+4°C |
|||
5 x 50 ml |
+4°C |
|||
79651 |
Colorimetric HRP substrate |
5 x 10 ml |
+4°C |
|
|
Transparent 96-well microplate |
5 x 1 |
+4°C |
Jiang, T., et al. Role of IL-2 in Cancer Immunotherapy. OncoImmunology, 2016. 5(6): e1163462