IL-2 Promoter Luciferase Reporter Lentivirus
The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-2 promoter (Figure 1). After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity.
Figure 1. Schematic of the lenti-vector used to generate the IL-2 promoter luciferase reporter lentivirus
• Jurkat cells (ATCC #TIB-152)
• Anti-CD3 agonist antibody (BPS Bioscience, #71274)
• Anti-CD28 agonist antibody (BPS Bioscience, #100182)
• Jurkat growth medium (Thaw Medium 2, BPS Bioscience, #60184)
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
• One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer