KIR3DL3/IL-2 Luciferase Reporter Jurkat Cell Line

Catalog #

78322

$11,250 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant Jurkat cell line expressing firefly luciferase under the control of an IL-2-responsive promoter, and with constitutive expression of human KIR3DL3 (Killer Cell Immunoglobulin Like Receptor, Three Ig Domains and Long Cytoplasmic Tail 3; GenBank accession #BC143802.1 corresponding to KIR3DL3*00402 allele).

Interested in screening and profiling inhibitors, blocking antibodies, or activators of KIR3DL3 without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Killer cell immunoglobulin-like receptor 3DL3, CD158 antigen-like family member Z, Killer cell inhibitory receptor 1, CD_antigen: CD158z, CD158Z, KIR3DL7, KIRC1

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Product Data Gallery

Dose-dependent luciferase activity in KIR3DL3/IL-2 Luciferase Reporter Jurkat Cell Line in response to anti-HHLA2(B7-H7) antibody when co-cultured with B7-H7(HHLA2)/TCR Activator CHO Cell Line in the presence of TCR agonist anti-CD28.

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat cells (clone E6-1), Human T lymphoblast, suspension

Species

Human

Supplied As

Each vial contains >2 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2P	BPS Bioscience #78354

Materials Required for Cellular Assay

Name	Ordering Information
Anti-HHLA2(B7-H7) antibody¹	Bahatt, RS, et al., 2021 (see references)
Growth Medium 2P	BPS Bioscience #78354
Assay Medium: Thaw Medium 2	BPS Bioscience #60184
B7-H7(HHLA2)/TCR Activator CHO Cell Line	BPS Bioscience #78321
Anti-CD28 agonist antibody	BPS Bioscience #100186
96-well tissue culture treated, white clear-bottom assay plate	Corning #3610
ONE-Step™ luciferase assay system	BPS Bioscience #60690
Luminometer

UniProt #

Q8N743, P60568

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

HHLA2 (B7-H7) mediates an immune-stimulatory signal via TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H) in naïve T cells while it delivers an immune-inhibitory signal through KIR3DL3 in activated T cells and Natural Killer (NK) cells.

References

1. Bahatt RS, et. al., Cancer Immunol Res. 2021 Feb;9(2):156-169.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2P (BPS Bioscience #78354): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.25 μg/ml of Puromycin and 1 mg/ml Geneticin.

Applications

Characterize the biological activity of KIR3DL3 interaction with B7-H7
Screen for anti-B7-H7 antibodies in co-culture with B7-H7-expressing cells

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy, NK

Regulatory Status

Research Use Only

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KIR3DL3/IL-2 Luciferase Reporter Jurkat Cell Line

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