B7-H7 (HHLA2)/TCR Activator CHO Cell Line

Catalog #

78321

$11,650 *

Size: 2 vials

Qty

Bulk/Custom

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Description

Recombinant CHO-K1 cells stably expressing human B7-H7 (also known as HHLA2: HERV-H LTR-associating protein 2, GenBank accession #NM_007072) and an engineered T cell receptor (TCR) activator. B7-H7 mediates an immune-stimulatory signal via TMIGD2 (Transmembrane and immunoglobulin domain containing 2) in naïve T cells while delivering an immune-inhibitory signal through KIR3DL3 in activated T cells and Natural Killer (NK) cells.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, B7y, B7H7, B7-H5, B7-H7

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Product Data Gallery

Activation of KIR3DL3/IL-2 Luciferase Reporter Jurkat cells in response to increasing doses of anti-HHLA2 antibody when co-cultured with B7-H7 (HHLA2)/TCR Activator CHO cells in the presence of TCR agonist anti-CD28.

TMIGD2/NFAT Luciferase Reporter Jurkat Cell Line activity in response to increasing doses of anti-HHLA2 antibody when co-cultured with B7-H7 (HHLA2)/TCR Activator CHO cells.

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3A	BPS Bioscience #60188

Materials Required For Cellular Assay

Name	Ordering Information
Anti-HHLA2(B7-H7) antibody¹	Bahatt RS, 2021 (see references)
Growth Medium 3A	BPS Bioscience #60188
Assay Medium: Thaw Medium 2	BPS Bioscience #60184
KIR3DL3/IL-2 Luciferase Reporter Jurkat Cell Line	BPS Bioscience #78322
TMIGD2/NFAT Luciferase Reporter Jurkat Cell Line	BPS Bioscience #78323
Anti-CD28 Agonist Antibody	BPS Bioscience #100186
96-well tissue culture treated, white clear-bottom assay plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Genbank #

NM_007072

UniProt #

Q9UM44

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

References

Bahatt RS, et. al., Cancer Immunol Res. 2021 Feb;9(2):156-169.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3A (BPS Bioscience #60188): F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml of Geneticin and 500 μg/ml of Hygromycin.

Media Required for Cellular Assay
Thaw Medium 2 (BPS Bioscience #60184): RPMI1650 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Applications

Characterize the biological activity of KIR3DL3 or TMIGD2 interactions with B7-H7
Screen for B7-H7 antibodies

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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B7-H7 (HHLA2)/TCR Activator CHO Cell Line

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