TMIGD2/NFAT Luciferase Reporter Jurkat Cell Line

Catalog #

78323

$10,340 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant Jurkat cell line expressing firefly luciferase under the control of an NFAT response element, and with constitutive expression of human TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H; NM_144615). Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of TMIGD2 without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

TMIGD2, Transmembrane and immunoglobulin domain-containing protein 2, CD28 homolog, Immunoglobulin and proline-rich receptor 1, IGPR-1, CD28H, IGPR1, UNQ3059/PRO9879

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Product Data Gallery

NFAT reporter activity in response to anti HHLA2(B7-H7) antibody when co-cultured with B7-H7/TCRa CHO cells.

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat cells (clone E6-1), Human T lymphoblast, suspension

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2A	BPS Bioscience #60190

Materials Required for Cellular Assay

Name	Ordering Information
Anti-HHLA2(B7-H7) antibody [1]	Bahatt RS, 2021 (see references)
Growth Medium 2A	BPS Bioscience #60190
Assay Medium: Thaw Medium 2	BPS Bioscience #60184
B7-H7(HHLA2)/TCR Activator CHO Cell Line	BPS Bioscience #78321
96-well tissue culture treated, white, clear-bottom assay plate	Corning #3610
ONE-Step™ luciferase assay system	BPS Bioscience #60690
Luminometer

UniProt #

Q96BF3

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

HHLA2 (B7-H7) mediates an immune-stimulatory signal via TMIGD2 in naïve T cells while it delivers an immune-inhibitory signal through KIR3DL3 (Killer Cell Immunoglobulin Like Receptor, Three Ig Domains and Long Cytoplasmic Tail 3) in activated T cells and Natural Killer (NK) cells.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2A (BPS Bioscience #60190): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 μg/ml of Hygromycin and 1 mg/ml Geneticin.

Applications

Characterize the biological activity of TMIGD2 interaction with B7-H7
Screen for B7-H7 antibodies in co-culture with B7-H7-expressing cells

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only