IL-1β (Human) Colorimetric ELISA Detection Kit

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Catalog #

79771

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Description

The cytokine protein Interleukin-1β is a mediator in the inflammatory response and is a therapeutic target for autoinflammatory diseases such as Alzheimer’s disease and Schnitzler syndrome. The IL-1β (Human) Colorimetric Detection Kit is designed for detecting and quantifying human interleukin-1β in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IL-1β are incubated on the coated plate followed by detecting the captured IL-1 β with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Colorimetric

Supplied As

This kit comes in a convenient 96-well format, with capture and detection antibodies for IL-1β, streptavidin-labeled HRP, blocking buffer, IL-1β standard, and colorimetric HRP substrate for 96-well plates.

Materials Required But Not Supplied

PBS (Phosphate-buffered saline)
PBST (PBS with 0.05% Tween-20)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used without adding 1N HCl, but sensitivity of the assay will be reduced.

Format

96 Reactions:

Catalog #	Component	Amount	Storage
	Interleukin-1β capture antibody	20 µl	-80°C	Avoid freeze/ thaw cycles!
	Interleukin-1β detection antibody, biotinylated	5 µl	-80°C
	Human IL-1β standard (1 µg/ml)	20 µl	-80°C
80611	Streptavidin-HRP	5 µl	+4°C
79743	Blocking Buffer 3	50 ml	+4°C
79651	Colorimetric HRP substrate	10 ml	+4°C
79964	Transparent 96-well microplate	1	+4°C

5 x 96 Reactions:

Catalog #	Component	Amount	Storage
	Interleukin-1β capture antibody	5 x 20 µl	-80°C	Avoid freeze/ thaw cycles
	Interleukin-1β detection antibody, biotinylated	5 x 5 µl	-80°C
	Human IL-1β standard (1 µg/ml)	40 µl	-80°C
80611	Streptavidin-HRP	5 x 5 µl	+4°C
79743	Blocking Buffer 3	5 x 50 ml	+4°C
79651	Colorimetric HRP substrate	5 x 10 ml	+4°C
79964	Transparent 96-well microplate	5 x 1	+4°C

UniProt #

P01584

References

1. Sciaccaa, FL., et al. Interleukin-1B polymorphism is associated with age at onset of
Alzheimer’s disease. Neurobiology of Aging 2003. 24: 927-931.
2. Roerink, M., et al. Interleukin-1 as a mediator of fatigue in disease. Journal of
Neuroinflammation. 2017, 14:16

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cytokine

Regulatory Status

Research Use Only