Firefly Luciferase K562 Cell Line

Catalog #

78621

$1,900 *

Size: 2 vials

Qty

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Description

Recombinant K562 cells constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity in Firefly Luciferase K562 cells

Lack of anti-CD20 CAR-T cytotoxicity against Firefly Luciferase K562 Cell Line

Product Info

Storage and Usage

Citations

Host Cell Line

K562, a human myelogenous leukemia cell line derived from a patient with CML; suspension.

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2D	BPS Bioscience #79639
96-well Tissue Culture-treated White Clear-bottom Assay Plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Formulation

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience #60184):
RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2D (BPS Bioscience #79639):
RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 μg/ml of Hygromycin B.

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The K562 cell line was established from a patient with chronic myelogenous leukemia (CML) in terminal blast crisis. The Firefly Luciferase K562 Cell Line does not express antigens CD19, CD20 or CD22, therefore it makes an excellent negative control in assays that measure specific killing by CAR-T cells targeting B cell antigens CD19, CD20, and CD22. It can also be used as a highly sensitive in vitro target for the natural killer assay. The signal generated by the firefly luciferase reporter is proportional to cell numbers.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Instructions for Use

See assay protocol for detailed instructions.

Applications

1. In vitro and in vivo Bioluminescence Imaging
2. Use as a negative control in CAR-T or NK co-culture killing assays

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only