NFAT Reporter – HEK293 Cell Line (PKC/ Ca2+ Pathway)

Catalog #

79298

$2,140 *

Size: 2 Vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NFAT Reporter – HEK293 cell line contains a firefly luciferase gene under the control of NFAT response element stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of phorbol 12-myristate 13-acetate (PMA) with ionomycin.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of NFAT-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Nuclear factor of activated T cells; T cell, PKC Ca 2+, Calcium PKC Pathway

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Product Data Gallery

NFAT Reporter (Luc) – HEK293 cell response to TCR Crosslinker Ionomycin with PMA

Inhibition of PKC by RO-31-8220 in PMA and Ionomycin induced NFAT reporter (Luc)-HEK293 cells

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293

Supplied As

Each vial contains ~2 X 10⁶ cells in 1 ml of 10% DMSO.

Materials Required But Not Supplied

• PMA (LC Laboratories #P1680) Prepare stock solution in DMSO.
• Ionomycin (Sigma #I3909) Prepare stock solution in DMSO.
• Assay medium: Thaw Medium 1 (BPS Bioscience #60187)
• Growth Medium 1B (BPS Bioscience #79531)
• 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
• RO-31-8220 (EMD Millipore #557520) or other PKC inhibitor
• ONE-Step Luciferase Detection Reagents (BPS Bioscience #60690) for measuring firefly luciferase activity.
• Luminometer

Mycoplasma Testing

The cell line has been screened using the PCR-based VenorGeM® Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

Background

The protein kinase c (PKC)/ Ca2+ response pathway leads to activation of the transcription factor nuclear factor of activator T cells (NFAT). NFAT is regulated by Ca2+ and the Ca2+/calmodulin-dependent serine phosphatase calcineurin. NFAT proteins are phosphorylated and reside in the cytoplasm in resting cells; upon stimulation, they are dephosphorylated by calcineurin, translocate to the nucleus, and induce gene expression.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1B (BPS Bioscience #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 μg/ml of Geneticin

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only