PD-L2 / TCR activator - CHO Recombinant Cell Line

Catalog #

79632

$9,400 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant CHO-K1 cells constitutively expressing human PD-L2 (Programmed Cell Death 1 Ligand 2 or CD273, GenBank accession #NM_025239) and an engineered T-cell receptor (TCR) activator.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC

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Product Data Gallery

Cell Characterization Using a PD-1 Neutralizing Antibody

Dose response of anti-PD-1 neutralizing antibody in PD-L2 / NFAT-Reporter Jurkat cells

FACS analysis of cell surface expression of PD-L2 in PD-L2 / TCR activator PD-L2 / TCR activator CHO cells

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1

Materials Required But Not Supplied

PD-1/NFAT reporter Jurkat cell line (BPS Bioscience #60535)
Assay medium: Thaw Medium 2 (BPS Bioscience #60184):
(RPMI1640 + 10% FBS + 1% Penicillin/Streptomycin)
Growth Medium 3A (BPS Bioscience #60188)
Anti-PD-1 neutralizing antibody (BPS Bioscience #71120)
96-well tissue culture-treated white clear-bottom assay plate
One-Step luciferase assay system (BPS Bioscience #60690)
Luminometer
Thaw Medium 3 (BPS Bioscience #60186):
Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079) and 1% Penicillin/Streptomycin (Hyclone SV30010.01)

UniProt #

Q9BQ51

Mycoplasma Testing

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

Background

The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found on most cancers, and the PD-1:PD-L1/2 interaction inhibits T-cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Cat. #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3A (BPS Cat. #60188): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml of Geneticin and 500 μg/ml of Hygromycin B to ensure recombinant expression.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only