TCR Activator Lentivirus (CMV Promoter/Puromycin) or (EF1A Promoter/Puromycin) or (EF1A Promoter/Hygromycin)
The TCR Activator Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a gene for a membrane-bound, engineered T cell receptor (TCR) activator driven by a CMV promoter or an EF1A promoter. After transduction, the expression of TCR activator can be monitored in the target cells.
This product has been cited 1 time.
• CHO-K1 cells (ATCC, #CCL-61)
• CHO growth medium or use BPS Thaw Medium 3 (BPS Bioscience, #60186)
• NFAT reporter (Luc) Jurkat cell line (BPS Bioscience, #60621)
• Jurkat growth medium or use BPS Thaw Medium 2 (BPS Bioscience, #60184)
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
• One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer
The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.