TCR Activator Lentivirus (CMV Promoter/Puromycin) or (EF1A Promoter/Puromycin) or (EF1A Promoter/Hygromycin)

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Catalog #
79894
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Description

The TCR Activator Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a gene for a membrane-bound, engineered T cell receptor (TCR) activator driven by a CMV promoter or an EF1A promoter. After transduction, the expression of TCR activator can be monitored in the target cells.

 

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Product Info
Storage and Usage
Citations1
Supplied As
Two vials (500 μl x 2) of TCR Activator Lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Materials Required But Not Supplied

• CHO-K1 cells (ATCC, #CCL-61)
• CHO growth medium or use BPS Thaw Medium 3 (BPS Bioscience, #60186)
NFAT reporter (Luc) Jurkat cell line (BPS Bioscience, #60621)
• Jurkat growth medium or use BPS Thaw Medium 2 (BPS Bioscience, #60184)
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.