SRE Reporter - HEK293 Recombinant Cell Line (ERK Pathway)

Catalog #

60406

$2,505 *

Size: 2 vials

Qty

Bulk/Custom

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Description

The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylates and activates ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.

The SRE Reporter – HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. This cell line is validated for the response to the stimulation of EGF or serum and to the treatment of inhibitors of ERK signaling pathway.

Interested in screening and profiling inhibitors or activators of MAPK/ERK signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 2 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

SRE cell line, MAPK reporter, ERK reporter, HEK

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Product Data Gallery

EGF or serum induced the expression of SRE reporter in SRE Reporter – HEK293

Dose response of SRE Reporter – HEK293 cells to EGF

U0126 blocked EGF-induced SRE reporter activity

U0126 inhibition dose response curve

Product Info

Storage and Usage

Citations2

Host Cell Line

HEK293

Species

Human

Supplied As

Each vial contains ~1.5 X 10⁶ cells in 1 ml of 10% DMSO.

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1B	BPS Bioscience #79531

Materials Required for Cellular Assay

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1B	BPS Bioscience #79531
Recombinant human EGF	BPS Bioscience #90201-1
U0126: Inhibitor of ERK pathway (MEK inhibitor). Prepare stock solution of U0126 in DMSO.	BPS Bioscience #27012
Assay Medium 1B	BPS Bioscience #79617
96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
ONE-Step™ luciferase assay system	BPS Bioscience #60690
Luminometer

UniProt #

P27361, P28482

Mycoplasma Testing

The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.

References

1. Wong, K.K. (2009) Recent developments in anti-cancer agents targeting the Ras/Raf/ MEK/ERK pathway. Recent Pat. Anticancer Drug Discov. 4(1):28-35.
2. Treisman, R. (1992) The serum response element. Trends Biochem. Sci. 17(10): 423-426

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% nonessential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1B (BPS Bioscience #79531): MEM medium supplemented with 10% FBS, 1% nonessential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin

Assay Medium 1B (BPS Bioscience #79617): MEM medium supplemented with 0.5% FBS, 1% nonessential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Applications

• Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
• Screen for activators or inhibitors of the MAPK/ERK signaling pathway.

Shipping Temperature

-80°C

Notes

License Disclosure

Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide

Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

60406

SDS

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Certificate of Analysis (CoA)

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SRE Reporter - HEK293 Recombinant Cell Line (ERK Pathway)

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