SRE eGFP Reporter - HEK293 Cell Line (ERK Pathway)
Recombinant HEK-293 cells expressing enhanced green fluorescent protein (eGFP) under the control of SRE responsive elements. This cell line is validated for its response to EGF or serum stimulation and to treatment with inhibitors of ERK signaling pathway.
Interested in screening and profiling inhibitors, blocking antibodies, or activators of ERK signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Materials Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 1 | BPS Bioscience #60187 |
| Growth Medium 1J | BPS Bioscience #79552 |
Materials Required for Cellular Assay
| Name | Ordering Information |
| Thaw Medium 1 | BPS Bioscience #60187 |
| Growth Medium 1B | BPS Bioscience #79552 |
| Assay Medium 1B | BPS Bioscience #79617 |
| Recombinant human EGF | BPS Bioscience #90201 |
| U0129 (MEK inhibitor) | BPS Bioscience #27012 |
| 24-Well Tissue Culture Treated Plate | |
| Round-Bottom 96 well plates or Round-Bottom Tubes | |
| Flow Cytometry Cell Staining Buffer | |
| Flow Cytometer or Fluorescence Microscope |
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1J (BPS Bioscience #79552): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 500 µg/ml of Geneticin/G418
Assay Medium 1: Thaw Medium 1 (BPS Bioscience #60187)
Assay Medium 1B (BPS Bioscience #79617): MEM medium supplemented with 0.5% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
The cell line has been screened to confirm the absence of Mycoplasma species.
The MAPK/ERK signaling pathway is a key regulator of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylates and activates ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The Ternary Complex Factors (TCFs), including Elk1, are among the best-characterized transcription factor substrates of ERK1/2. When phosphorylated by ERK1/2, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
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