CD38 Inhibitor Screening Assay Kit (Hydrolase Activity)

Catalog #

79672

$1,120 *

Size: 384 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD38 Inhibitor Screening Assay Kit (Hydrolase Activity) is designed to measure the glycohydrolase activity of CD38 for screening and profiling applications.

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Synonyms

cluster of differentiation 38, cyclic ADP ribose hydrolase, activity assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Supplied As

The CD38 assay kit comes in a convenient high-throughput 384-well format, with purified recombinant CD38 enzyme, its substrate N6-etheno-NAD (ε-NAD), and CD38 assay buffer for 384 enzyme reactions.

Materials Required But Not Supplied

Adjustable micropipettor and sterile tips

Fluorescent microplate reader

Rotating or rocker platform

Format

Catalog Number	Reagent	Amount	Storage
71277	CD38, His-Tag (Human), HiP™	12 µg	-80°C	Avoid mulitple freeze/thaw cycles!
	4x CD38 hydrolase buffer	10 ml	-20°C
	CD38 substrate (ε-NAD)	200 µl	-20°C
	Apigenin (50 mM DMSO)	10 µl	-20°C
79961	Black 384-well plate	1

UniProt #

P28907

Background

CD38, a differentiation antigen of B lymphocytes, is a type II integral membrane protein. It is also known as ADP-ribosyl cyclase and nicotinamide adenine dinucleotide (NAD) glycohydrolase. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in various cells, including pancreatic β cells. The major enzymatic activity of CD38 is the hydrolysis of NAD. CD38 is a prognostic biomarker for acute B lymphoblastic leukemia.

References

Wei, W., et al., World J. Biol. Chem. 2014. 5(1):58-67.

Storage/Stability

Up to 6 months from date of receipt, when stored as recommended.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only