Anti-CD20 CAR Lentivirus (Clone Leu-16 ScFv-CD8-4-1BB-CD3ζ)

Catalog #

78606

$1,095 *

Size: 50 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The anti-CD20 CAR lentiviruses are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv (single-chain variable fragment) of anti-CD20 (clone Leu-16) linked to a 2^nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1).

Schematic of the lenti-vector used to generate the anti-CD20 CAR lentivirus.

Construct diagram showing components of the anti-CD20 CAR.

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Synonyms

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD_antigen: CD20, MS4A1

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Product Data Gallery

Product Info

Storage and Usage

Citations

Supplied As

50 µl of anti-CD20 CAR at a titer ≥ 10⁸ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
PBMC, Frozen	BPS Bioscience #79059
Human Interleukin-2	BPS Bioscience #90184
EasySep™ Human CD4+ T Cell Isolation Kit	Stemcell technologies #17952
EasySep™ Human CD8+ T Cell Isolation Kit	Stemcell technologies #17953
Human CD3/CD28/CD2 T Cell Activator	Stemcell technologies #10970
Biotinylated Protein L	Genscript #M00097
PE-Streptavidin	Biolegend #405203
IFN-γ (Human) Colorimetric ELISA Detection Kit	BPS Bioscience #79777
CD20/Firefly Luciferase CHO Cell Line	BPS Bioscience #78620
Firefly Luciferase CHO Cell Line	BPS Bioscience #79725
Firefly Luciferase Raji Cell Line	BPS Bioscience #78622
Firefly Luciferase K562 Cell Line	BPS Bioscience #78621
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690

Formulation

The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM.

Recommended CD4+CD8+ T Cell Medium: StemSpan SFEM (Stemcell Technologies, #09650) supplemented with 10% heat-inactivated FBS (Life Technologies, #10082147), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01), plus 10 ng/ml IL-2 (BPS Bioscience, #90184).

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Applications

Ideal as a positive control for anti-CD20 CAR evaluation in T cells; useful for transduction optimization.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Biosafety
The lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Immunotherapy, CAR-T

Regulatory Status

Research Use Only

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Anti-CD20 CAR Lentivirus (Clone Leu-16 ScFv-CD8-4-1BB-CD3ζ)

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