CD20 Positive Cell Isolation Kit

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Catalog #

82132

As low as $70 *

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD20 Positive Cell Isolation Kit is designed to magnetically separate CD20-expressing cells from a complex immune cell population. This kit is optimized for the isolation of CD20 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, the CD20 positive cells are immobilized along the side of the tube while CD20-negative cells remain in suspension and can be easily removed.

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Synonyms

B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1, B-lymphocyte antigen CD20

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Product Data Gallery

Flow cytometry analysis of CD20+ cells pre- and post-isolation.

Product Info

Storage and Usage

Citations

Supplied As

This kit provides enough reagents and materials for isolation of CD20+ cells from up to 1 x 10⁸ or 1 x 10⁹ PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC amounts.

Materials Required But Not Supplied

Peripheral blood mononuclear cells (PBMCs) (BPS Bioscience #79059)
Cell Isolation Magnetic Tube Rack (BPS Bioscience #78571)
Centrifuge
15- or 50-ml tubes
Cell counter

Format

1 x 10⁸Cells

Catalog #	Name	Amount	Storage
	Cell Isolation Magnetic Beads	200 µl	+4°C
	CD20 Cell Isolation Antibody	40 µl	-20°C
78563	5x Cell Isolation Buffer	25 ml	+4°C

1 x 10⁹Cells

Catalog #	Name	Amount	Storage
	Cell Isolation Magnetic Beads	2 ml	+4°C
	CD20 Cell Isolation Antibody	400 µl	-20°C
78563	5x Cell Isolation Buffer	250 ml	+4°C

UniProt #

P11836

Background

B cells are critical immune cells for functions such as antibody production, support of other mononuclear and antigen-presenting cells, and they influence inflammatory pathways. CD20 is a marker of certain B cell subsets, which include B cell blasts, B cell malignancies, pre-B cells, naïve, mature, and activated B cells. There is also a small subset of CD20⁺ T cells in PBMCs. In PBMCs derived from healthy individuals, about 6-23% of the cells are CD20⁺ cells.

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CD20 Positive Cell Isolation Kit

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