Anti-CD20 IgG Antibody, Biotin-labeled

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Catalog #

101207

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Description

Biotinylated anti-CD20 IgG antibody is a purified recombinant antibody which recognizes human CD20 antigen. This antibody has been tested for specific binding to purified human CD20 protein.

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4-20% SDS-PAGE Coomassie Staining

CD20:anti CD20-Biotin Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Host Species/Expression System

Heavy chain (HC) and Light chain (LC) co-expressed in HEK293

Purity

≥90%

Purification

Protein A affinity

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Heavy Chain: 53 kDa; Light Chain 23 kDa

UniProt #

P11836

Label

More than 90% biotinylation

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

Functional validation: The antibody was validated by ELISA using a purified CD20 antigen. The CD20 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CD20 antibody (BPS Bioscience #101207), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Applications

This product is for research use only. It is not suitable for human diagnostic or therapeutic use.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Anti-CD20 IgG Antibody, Biotin-labeled

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