This anti-CD20 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested in flow cytometry assay for specific binding to human CD20 on the surface of CD20 CHO Recombinant Cell Line (BPS Bioscience #79624).
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Synonyms
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1
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Product Data Gallery
Cell surface expression of CD20 in CD20 CHO Cell Line
Protein A affinity chromatography from HEK293 supernatants.
Format
Aqueous buffer solution
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain protein stabilizer.
Concentration
The concentration will vary with each lot; the exact value will be provided with each shipment.
UniProt #
P11836
Label
PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa).
Storage/Stability
At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Assay Conditions
One million CD20 CHO recombinant cells (BPS Bioscience #79624) were blocked and stained with 1 µg of anti-CD20 antibody, PE-labeled (BPS Bioscience #101672) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD20 CHO recombinant cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.
Applications
Useful for studying expression of CD20 on the cell surface in flow cytometry assay. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.
Shipping Temperature
+4°C (gel packs)
Cross Reactivity
This antibody recognizes human CD20. It has not been tested with other species.
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