CD20, FLAG-Tag Recombinant

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101572

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Description

Recombinant human CD20, also known as membrane spanning 4-domains A1 (MS4A1), full length, encompassing amino acids 1-297(end). This construct contains a C-terminal FLAG-tag. This recombinant protein was affinity purified.

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Synonyms

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD20: Anti-CD20 Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Construct

CD20 (1-297(end)-FLAG)

Host Species/Expression System

HEK293

Purity

≥85%

Format

Aqueous buffer solution

Formulation

50 mM HEPES (pH 8), 150 mM NaCl, 10% (vol/vol) glycerol, 0.1% DDM, 0.01% CHS, and 100 µg/ml FLAG peptide

34kDa

Amino Acids

1-297(end)

Genbank #

NM_021950.3

UniProt #

P11836

Tag(s)

C-terminal FLAG-tag

Storage/Stability

At least 6 months at -80°C

Assay Conditions

The protein was validated by measuring its binding to Anti-CD20 antibody, Biotin-labeled (BPS Bioscience #101207) in biochemical binding (ELISA) assay. The CD20 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-CD20 antibody, biotin-labeled, serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with HRP Streptavidin (BioLegend #405210), washed, and incubated with Colorimetric HRP substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

101572 Lot 230109

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CD20, FLAG-Tag Recombinant

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