Anti-CD20 CAR-T Cells

Catalog #

78611

$1,600 *

Size: 1 vial

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The anti-CD20 CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-CD20 CAR Lentivirus (CD20 ScFv-CD8-4-1BB-CD3ζ; BPS Bioscience #78606). These ready-to use CAR-T cells express an anti-CD20 CAR consisting of the ScFv (Single chain fragment variable) of anti-CD20 (clone Leu-16) linked to a 2^nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1).

These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Figure 1: Construct diagram showing components of the anti-CD20 CAR expressed in anti-CD20 CAR-T cells.

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Synonyms

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1

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Product Data Gallery

Expression of anti-CD20 CAR in anti-CD20 CAR-T cells

IFN-g expression analysis using CD20 CHO as the target cells

Product Info

Storage and Usage

Citations

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of CryoStor® CS10 (Stemcell Technologies)

Materials Required But Not Supplied

Name	Ordering Information
Human Interleukin-2	BPS Bioscience #90184
Human CD3/CD28/CD2 T Cell Activator	Stemcell technologies #10970
Biotinylated Protein L	Genscript #M00097
PE-Streptavidin	Biolegend #405203
CD20 CHO Recombinant Cell Line (High Expression)	BPS Bioscience #79624-H
IFN-g (Human) Colorimetric ELISA Detection Kit	BPS Bioscience #79777

Recommended anti-CD20 CAR-T Cell Medium: TCellM™ (BPS Bioscience #78753) supplemented with 10 ng/ml Interleukin-2 (BPS Bioscience #90184).

UniProt #

P11836

Mycoplasma Testing

The cells have been screened to confirm the absence of Mycoplasma species.

Background

CD20 (also known as MS4A1) is a glycosylated phosphoprotein expressed on the cell surface of B cells. CD20 is a highly attractive target antigen for immunotherapy because it is highly expressed in more than 90% of patients with B-cell lymphoma. First approved in 1997, Rituximab (Rituxan) is a chimeric monoclonal antibody targeting CD20 and has been classified by the World Health Organization as an “Essential Medicine”. Since then, additional monoclonal antibodies against CD20 have been approved or are being tested in clinical trials for the treatment of multiple sclerosis (MS), chronic lymphocytic leukemia (CLL), follicular lymphoma, diffuse large B cell lymphoma (DLBCL), rheumatoid arthritis, non-Hodgkin’s lymphoma, systemic lupus erythematosus, and myalgic encephalomyelitis (chronic fatigue syndrome). More recently, anti-CD20-CD19 bispecific CAR-T cells have been developed to address concerns over potential relapse in cancer patients.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Applications

Use as positive control for anti-CD20 CAR-T cells
Screen modulators of anti-CD20 CAR-T cytotoxicity
Design and optimize co-culture cytotoxicity assays

Shipping Temperature

-80°C

Notes

Biosafety
The anti-CD20 CAR-T cells are produced with the third generation SIN (self-inactivation) lenti vector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle.

Troubleshooting Guide
Visit Cell Line FAQs for more information.
For further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Donors have been screened and determined negative for:

Hepatitis B (anti-HBc EIA, HBsAg EIA)
Hepatitis C (anti-HCV EIA)
Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)
Human T-Lymphotropic Virus (HTLV-I/II)
HIV-1/HCV/HBV
West Nile Virus
Trypanasoma cruzi

Note: Testing cannot guarantee that any sample is completely virus-free. These cells should be treated as potentially infectious and appropriate biological safety level 2 precautions should be used.

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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Anti-CD20 CAR-T Cells

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