CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit

Catalog #: 72009
Size: 96 reactions
$1,040
Background:
The activation of naïve T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in an inhibitory signal and prevents T-cell activation. CTLA4:B7-1 interaction is an important drug target for the regulation of the host’s response to cancer.
Description:

The CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CTLA4 is coated on a 96-well plate. Next, B7-1 is incubated with CTLA4 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

UniProt
P33681
Synonym(s):
Cytotoxic T-lymphocyte-associated protein 4, CD152, CTLA-4 Counter-Receptor, B7.1, CD80
Supplied As:
This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified CTLA4, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
This kit is useful for screening for inhibitors of B7-1 binding to CTLA4.
Reference(s):
1. Linsley, P.S., et al. J. Exp. Med. 1991, 174(3): 561-569.
2. Hurwitz, A.A., et al. Canc. Res. 2000, 60: 2444-2448.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy/Cell Surface Receptor/Single-pass Transmembrane Protein
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

72009.pdf