CD47:SIRP-γ[Biotinylated] Inhibitor Screening Assay Kit

Catalog #: 72059
Size: 96 reactions
$1,040
Background:
Signal-regulatory protein gamma (SIRP-λ), also known as CD172g, is a cell surface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, is ubiquitously expressed on the surface of all cells and has been found to be overexpressed in some cancers. CD47 binding to SIRP-λ has been shown to be important for T cell trans-endothelial migration (TEM).
Description:
The CD47:SIRP-γ[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-γ signaling. The key to this kit is the high sensitivity of detection of biotin-labeled SIRP-γ through streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate. Next, SIRP-γ is incubated with CD47 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Synonym(s):
leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein (IAP), antigenic surface determinant protein OA3, MER6, Signal regulatory protein-gamma, signal regulatory protein beta 2, and CD172g
Format:

Storage / Stability:
This kit comes in a convenient 96-well format, with biotin-labeled SIRP-γ (CD172g), purified CD47, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. Stable for one year from date of receipt when stored as directed.
Application(s):
This kit is useful for screening for inhibitors of SIRP-γ binding to CD47.
Reference(s):
1. Stefanidakis, et al., Blood. 2008, 112(4):1280-1289

2. Sick, E., et al. Br J Pharmacol. 2012, 167(7):1415-1430.

Scientific Category:
Immunotherapy, Cell Surface Receptors
Product Type:
Assay Kit
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Product Datasheets

72059.pdf