IDO1 Inhibitor Screening Assay Kit

Catalog #: 72021
Size: 96 Reactions
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

The IDO1 Inhibitor Screening Assay Kit is designed to measure IDO1 enzyme inhibition. The IDO1 Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=321 nm.

Indoleamine 2,3-dioxygenase 1, activity assay kit
Supplied As:
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.
DMSO >0.5%, strong acids or bases, ionic detergents, high salt


Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Useful for the study of IDO1 enzymology, screening inhibitors, and selectivity profiling.

1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
2. Seegers, N., et al. J Biomol Screen. 2014; 19(9): 1266-74.

Application Reference(s):
1. Synthesis and Molecular Modeling Studies of N'-Hydroxyindazolecarboximidamides as Novel Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors (2017)

Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets