IDO1, His-Avi-Tag, Biotin Labeled Recombinant

Catalog #

79081

$455 *

Size: 50 μg

Qty

Bulk/Custom

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Description

Human IDO1, also known as Indoleamine 2,3-dioxygenase 1, GenBank Accession No. NM_002164, a.a. 2-403(end) with an N-terminal His-Avi-Tag, expressed in an E. coli cell expression system. MW = 48 kDa.

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Synonyms

Indoleamine 2,3-dioxygenase 1, IDO1, IDO1, Indoleamine 2,3-Dioxygenase 1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 20% glycerol

48 kDa

Amino Acids

2-403

Genbank #

NM_002164

UniProt #

P14902

Tag(s)

His-Avi-Tag

References

1. Lob, S. et al., Cancer Immunol. Immunother. 2009; 58(1): 153-157.

2. Liu, X. et al., Blood. 2010; 115(17): 3520-3530.

3. Flick, H.E., et al., Int. Nat. J. of Tryptophan Res. 2013; 6: 35-45.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

The assay was performed by UV absorption using a recombinant IDO1 or TDO2 and L-Tryptophan substrate. The UV absorption signal at 321 nm is correlated with the amount of N-formylkynurenine reaction product of reaction. All of the reactions were conducted at room temperature. The 100 µl reaction mixture in IDO or TDO Assay Buffer contains ~40 nM IDO1 (200 ng/well for 71182 or 210 ng/well for 79081) or varying concentrations (0-320 nM) of IDO1, 900 tryptophan, and the coupled reaction components. The reaction mixture incubated for 165 min respectively while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals were measured using a Tecan Infinite M1000 plate reader.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Notes

The aggregation state of this product has been confirmed to be <10%.

Warnings

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Regulatory Status

Research Use Only