EGFR(T790M/L858R) Kinase Assay Kit
The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cellsurface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. EGFR(T790M/L858R) is an EGFR activating and drug resistance mutant that can be found in non-small-cell lung cancer cells. The EGFR(T790M/L858R) Kinase Assay Kit is designed to measure EGFR (T790M/L858R) Kinase activity for screening and profiling applications using KinaseGlo® MAX as a detection reagent.
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This product has been cited 5 times.
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
Catalog Number |
Reagent | Amount | Storage | |
40350 | EGFR (T790M/L858R) | 2 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
79334 | 5x Kinase Buffer 1 | 1.5ml | -20°C | |
79686 | ATP (500 µM) | 100µl | -20°C | |
40217 | PTK substrate Poly (Glu:Tyr 4:1) (10 mg/ml) |
100µl | -20°C | |
79696 | 96-well plate, white | 1 | Room Temp. |
Nakamura, J.L. Expert Opin. Ther. Targets 11(4):463-472 (2007)