EGFR (T790M, L858R), GST-tag, His-tag Recombinant

Catalog #

40350

$355 *

Size: 10 µg

Qty

Bulk/Custom

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This product has been cited 2 times.

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Synonyms

EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations2

Species

Human

Construct

EGFR (T790M, L858R) (GST-668-1210-His)

Mutation

T790M, L858R

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 300 mM imidazole.

88 kDa

Amino Acids

668-1210

Specific Activity

680 pmol/min/μg.

Genbank #

NM_005228

UniProt #

P00533

Tag(s)

C-terminal His tag, N-terminal GST-tag

References

1. Adachi,S. et al., Cancer Res. 67 (13), 6493-6501 (2007)
2. Rosano,L. et al., Cancer Res. 67 (13), 6351-6359 (2007).

Application References:
1. EGFR inhibition studies by hybrid scaffolds for their activity against ovarian cancer (2016)

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in a kinase buffer containing 2 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40350 Lot 171010-3

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EGFR (T790M, L858R), GST-tag, His-tag Recombinant

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