Anti-FcGR3A-Anti-EGFR Bispecific Antibody

Catalog #

101535

$335 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Anti-FcGR3A-anti-EGFR bispecific IgG antibody, which recognizes FcGR3A (also known as CD16A) and EGFR antigen binding proteins. This bispecific antibody has been tested for specific binding affinity to purified human FcGR3A and EGFR recombinant protein.

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Synonyms

CD16-II, CD16a antigen, FcR-10, IgG Fc receptor III-2, FCG3, IGFR3, CD16A, FcγRIIIA, FCGRIIIA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Binding Assay

Activation of luciferase in FcGR3A/NFAT-Jurkat Reporter cells by Anti-FcGR3A-Anti-EGFR IgG in the presence of EGFR-CHO cells

Product Info

Storage and Usage

Citations

Species

Human

Clonality

Comprised of two monoclonal antibodies.

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Heavy Chain: 76 kDa; Light Chain: 23 kDa + glycans

Glycosylation

This antibody runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt #

FcGR3A: P08637; EGFR: P00533

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

Functional validation: The antibody was validated by measuring anti-FcGR3A-anti-EGFR binding to FcGR3A and EGFR antigens in ELISA assay. The FcGR3A protein (BPS Bioscience #79013) and EGFR protein were coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-FcGR3A-anti-EGFR antibody (BPS Bioscience #101535), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying the binding of FcGR3A and EGFR in ELISA and in cellular assays.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

101535 Lot 220809

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Anti-FcGR3A-Anti-EGFR Bispecific Antibody

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