EGFR Kinase Assay Kit

Catalog #

40321

$560 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. The EGFR Kinase Assay Kit is designed to measure EGFR Kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against EGFR? Check out our Kinase Screening Services.

This product has been cited 50 times.

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Synonyms

EGFR, ErbB-1, HER1

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Product Data Gallery

Product Info

Storage and Usage

Citations50

Assay Kit Format

Luminescent

Supplied As

The EGFR Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40187	EGFR (wild type)	2 µg	-80°C	*Avoid* *multiple* *freeze/* *thaw* *cycles!*
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
40217	PTK substrate Poly (Glu:Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.