Untransduced T Cells

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Catalog #

78170

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Description

The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation, spinoculation (without lentivirus), and expansion. These T cells are meant to be negative controls in experiments using lentivirus-transduced primary CAR-T cells.

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Product Info

Storage and Usage

Citations

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of CryoStor® CS10)

Materials Required But Not Supplied

Recommended T Cell Medium: TCellM™ (BPS Bioscience #78753) supplemented with 10 ng/ml Interleukin-2 (BPS Bioscience #90184).

Mycoplasma Testing

The cells have been screened to confirm the absence of Mycoplasma species.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Applications

Negative control for lentivirus-transduced CAR-T cells

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit Cell Line FAQs for more information. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Donors have been screened and determined negative for:- Hepatitis B (anti-HBc EIA, HBsAg EIA)- Hepatitis C (anti-HCV EIA)- Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)- Human T-Lymphotropic Virus (HTLV-I/II)- HIV-1/HCV/HBV- West Nile Virus- Trypanasoma cruziNote: Testing cannot guarantee that any sample is completely virus-free. These cells should be treated as potentially infectious and appropriate biological safety level 2 precautions should be used.

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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Untransduced T Cells

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