Human T Cell Isolation Kit
The Human T Cell Isolation Kit is designed to magnetically separate T cells from a complex immune cell population. This kit is optimized for the negative selection of CD3+ T cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with a mix of antibodies followed by conjugation to magnetic beads. They are then placed on a magnet for quick and easy separation. When placed on the magnet, non-T cells will be immobilized along the side of the tube while untouched T cells will remain in suspension for downstream use.
- Peripheral Blood Mononuclear Cells, Frozen (BPS Bioscience #79059)
- Thaw Medium 2 (BPS Bioscience #60184)
- Centrifuge
- 5-, 15-, and 50-ml centrifuge tubes
| Catalog # | Name | Amount | Storage |
| Cell Isolation Magnetic Beads | 500 µl | 2-8°C | |
| T Cell Isolation Antibody Cocktail | 500 µl | 2-8°C | |
| 78563 | 5x Cell Isolation Buffer | 25 ml | 2-8°C |
T cells are important immune cells that have a variety of functions. T cells can wipe out infected or cancerous cells. They also direct the immune response by helping B lymphocytes to eliminate invading pathogens. CD3 is a common T cell antigen while CD4 and CD8 are antigens present on the surface of T-helper and cytotoxic T cells, respectively. In PBMCs derived from healthy individuals, 45-70% of the cells are T cells.
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