Anti-Mesothelin CAR Lentivirus (P4 ScFv-CD8-4-1BB-CD3ζ)

Catalog #

78703

$1,150 *

Size: 50 µl

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Description

The Anti-mesothelin CAR lentiviruses are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv (single-chain fragment variable) of anti-mesothelin (Clone P4) linked to a 2^nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1).

Figure 1. (A) Schematic of the lenti-vector used to generate the anti-mesothelin CAR lentivirus and (B) Construct diagram showing components of the anti-mesothelin CAR.

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Synonyms

CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, Megakaryocyte-potentiating factor (MPF), MSLN, MPF

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Product Data Gallery

The expression of anti-Mesothelin CAR in HEK293 cells transduced with anti-mesothelin CAR lentivirus

The expression of anti-Mesothelin CAR in T cells transduced with Anti-Mesothelin CAR lentivirus

IFNg expression analysis using Mesothelin CHO as the target cells

Product Info

Storage and Usage

Citations

Supplied As

50 µl of anti-mesothelin CAR at a titer ≥ 3x10⁸ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
PBMC, Frozen	BPS Bioscience #79059
Human Interleukin-2	BPS Bioscience #90184
EasySep™ Human CD4+ T Cell Isolation Kit	Stemcell Technologies #17952
EasySep™ Human CD8+ T Cell Isolation Kit	Stemcell Technologies #17953
Human CD3/CD28/CD2 T Cell Activator	Stemcell Technologies #10970
Biotinylated Human Mesothelin	BPS Bioscience #100291
PE-Streptavidin	Biolegend #405203
Mesothelin - CHO Recombinant Cell Line	BPS Bioscience #78132
IFN-g (Human) Colorimetric ELISA Detection Kit	BPS Bioscience #79777

Formulation

The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM.

UniProt #

Q13421

Background

Mesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently overexpressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy, including drug-conjugated antibodies and chimeric antigen receptor T cells (CAR-T Cells).

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Applications

Positive control for anti-mesothelin CAR evaluation in T cells; useful for transduction optimization.

Shipping Temperature

-80°C

Notes

Biosafety
The lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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Anti-Mesothelin CAR Lentivirus (P4 ScFv-CD8-4-1BB-CD3ζ)

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