Anti-Mesothelin CAR-T Cells

Catalog #

78729

$1,600 *

Size: 1 vial

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Anti-mesothelin CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-Mesothelin CAR Lentivirus (Mesothelin ScFv-CD8-4-1BB-CD3ζ; BPS Bioscience #78703). These ready-to use CAR-T cells express an anti-Mesothelin CAR consisting of the scFv (Single chain fragment variable) of anti-Mesothelin (clone P4) linked to a 2^nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1).

These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Figure 1: Construct diagram showing components of the anti-Mesothelin CAR expressed in anti-Mesothelin CAR-T cells.

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Synonyms

CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, Megakaryocyte-potentiating factor (MPF), MSLN, MPF, SMRP

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Product Data Gallery

Expression of anti-Mesothelin CAR in Anti-Mesothelin CAR-T cells

IFN-g expression analysis using Mesothelin CHO as the target cells

Product Info

Storage and Usage

Citations

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of CryoStor® CS10 (Stemcell Technologies)

Materials Required But Not Supplied

Name	Ordering Information
Human Interleukin-2	BPS Bioscience #90184
Human CD3/CD28/CD2 T Cell Activator	Stemcell Technologies #10970
Biotinylated Human Mesothelin	BPS Bioscience #100291
PE-Streptavidin	Biolegend #405203
Mesothelin- CHO Recombinant Cell Line	BPS Bioscience #78132
IFN-g (Human) Colorimetric ELISA Detection Kit	BPS Bioscience #79777

Recommended anti-Mesothelin CAR-T Cell Medium: TCellM™ (BPS Bioscience #78753) supplemented with 10 ng/ml Interleukin-2 (BPS Bioscience #90184).

UniProt #

Q13421

Mycoplasma Testing

The cells have been screened to confirm the absence of Mycoplasma species.

Background

Mesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently overexpressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy approaches, including drug-conjugated antibodies and chimeric antigen receptor T cells (CAR-T Cells).

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Applications

Use as positive control for anti-Mesothelin CAR-T cells
Screen modulators of anti-Mesothelin CAR-T cytotoxicity
Design and optimize co-culture cytotoxicity assays

Shipping Temperature

-80°C

Notes

Biosafety
The Anti-Mesothelin CAR-T cells are produced with the third generation SIN (self-inactivation) lenti vector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle.

Troubleshooting Guide
Visit Cell Line FAQs for more information.
For further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Donors have been screened and determined negative for:

Hepatitis B (anti-HBc EIA, HBsAg EIA)
Hepatitis C (anti-HCV EIA)
Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)
Human T-Lymphotropic Virus (HTLV-I/II)
HIV-1/HCV/HBV
West Nile Virus
Trypanasoma cruzi

Note: Testing cannot guarantee that any sample is completely virus-free. These cells should be treated as potentially infectious and appropriate biological safety level 2 precautions should be used.

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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Anti-Mesothelin CAR-T Cells

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