SRC, His-Tag Recombinant

Catalog #

40484

$395 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human SRC (SRC proto-oncogene, non-receptor tyrosine kinase), full length encompassing amino acids 2-536 (end). The construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

c-SRC, Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog, SRC1, p60-Src, ASV

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

SRC Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

SRC (His-2-536(end))

Host Species/Expression System

Sf9

Purity

≥85%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, variable imidazole, and 20% glycerol

61 kDa

Amino Acids

2-536(end)

Specific Activity

350 pmol/min/μg

Genbank #

NM_005417

UniProt #

P12931

Tag(s)

N-terminal His-tag

References

1. Finlay, D. and Vuori, K. Cancer Res. 67 (24), 11704-11711 (2007).
2 Vielreicher, M. et al. J. Biol. Chem. 282 (46), 33623-33631 (2007).

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was done according to SRC Assay Kit (BPS Bioscience #79680) with various amounts of SRC.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40484 Lot 220217-2

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