SRC Assay Kit

Catalog #

79680

$535 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

SRC is a member of the nonreceptor tyrosine kinases that plays a role in many cellular functions, including cell adhesion, growth, and differentiation. SRC has been implicated in diseases such as chronic kidney disease and metastatic bone disease. The SRC Assay Kit is designed to measure SRC activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

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Synonyms

c-SRC, Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog, SRC1

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The SRC Assay Kit comes in a convenient 96-well format, with enough purified recombinant SRC enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40484	SRC, His-tag	>1 µg	-80°C	*Avoid multiple freeze/ thaw cycles!*
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40217	Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P12931

References

1. Vidal, M., et al. Differing Src signaling levels have distinct outcomes in Drosophila; 2007, Cancer Res. 67(21): 10278—10285.
2. Wan, J. and Zhuang, S. Src Family Kinases in Chronic Kidney Disease; 2017, Amer. J. Physiol. Renal Physiol.313(3): F721–F728.

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay kit data sheet for detailed protocol

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only