SRC, GST-tag Recombinant

Catalog #

40483

$355 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human SRC (GenBank Accession No. NM_005417), full length with N-terminal GST-tag, MW = 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

●

Synonyms

c-SRC, Avian Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog, SRC1

●

Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥60%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT.

86 kDa

Amino Acids

full length

Specific Activity

55 pmol/min/μg

Genbank #

NM_005417

UniProt #

P12931

Tag(s)

N-terminal GST-tag

References

1. Finlay, D. and Vuori, K. Cancer Res. 67 (24), 11704-11711 (2007).
2 Vielreicher, M. et al. J. Biol. Chem. 282 (46), 33623-33631 (2007).

Storage/Stability

>6 months at –80°C.

Assay Conditions

50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 0.01% Brij-35, 200 μM ATP, 2 μM Tyr2 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

40483_Lot80205.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

SRC, GST-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY