Sphingosine kinase 1, His-tag

Catalog #: 40610
Size: 20 µg
$385
Species: Human
Host Species: Sf9 insect cells
MW: 46.9 kDa
Genbank #: NM_001142601.1
Tag(s): N-terminal His-tag
a.a: full length
Description:
Human Sphingosine kinase 1 or SphK1 (GenBank Accession No. AF200328), full length with N-terminal His-tag, MW=46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q9NYA1-1
Synonym(s):
SphK1, sphingosine kinase 1, SPK1
Specific Activity:
3,300 pmole/min/μg
Unit Definition:
A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Assay Conditions:
Assay was done in Kinase buffer containing 20 μM Sphingosine and 20 μM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Assay was done in Kinase buffer (40 mM Tris, pH 7.4, 20 mM MgCl2+, 0.1 mg/ml BSA) containing 20 μM Sphingosine and 20 μM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)

Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004).
2. Taha, TA., et al., FASEB J. 20(3):482-4 (2005).

Application Reference:
Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation (2014)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Lipids
Product Type:
Recombinant Protein
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Product Datasheets

40610_Lot140618.pdf