Sphingosine kinase 1, His-tag Recombinant

Catalog #

40610

$405 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human Sphingosine kinase 1, also known as SphK1 and SPK1, GenBank Accession No. AF200328, a.a. 1-384 (full length), with N-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=47 kDa.

This product has been cited 5 times.

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Synonyms

SphK1, sphingosine kinase 1, SPK1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations5

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole, 3 mM DTT and 20% glycerol.

47 kDa

Amino Acids

1-384 (full length)

Specific Activity

1900 pmole/min/μg

Genbank #

NM_001142601.1

UniProt #

Q9NYA1-1

Tag(s)

N-terminal His-tag

References

1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004).
2. Taha, TA., et al., FASEB J. 20(3):482-4 (2005).

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in Kinase buffer containing 1 mM DTT using 20 μM Sphingosine and 20 μM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Lipids

Regulatory Status

Research Use Only

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Datasheets/Protocols

40610 Lot 190719-2.pdf

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Sphingosine kinase 1, His-tag Recombinant

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