Mouse Sphingosine kinase 2, His-tag Recombinant

Catalog #

40614

$415 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Mouse SPHK2, also known as Sphingosine kinase 2 and SPK2, GenBank Accession No. NM_203280, a.a. 1-617 (full length) with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=70 kDa.

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Synonyms

SphK2, sphingosine kinase 2, SPK2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥60%

Supplied As

Aqueous buffer solution.

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3mM DTT and 50% glycerol.

70 kDa

Amino Acids

1-617 (full length)

Specific Activity

2 pmol/min/μg

Genbank #

NM_203280

UniProt #

Q9JIA7

Tag(s)

N-terminal His-tag

References

1. Zemann, B. et al., Blood.107(4):1454-8 (2006)
2. Maceyka, M. et al., J Biol Chem. 280(44):37118-29 (2005)

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in Kinase buffer containing 1 mM DTT using 20 μM Sphingosine and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Lipids

Regulatory Status

Research Use Only

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Datasheets/Protocols

40614 Lot 190523-2.pdf

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Mouse Sphingosine kinase 2, His-tag Recombinant

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