Mouse Sphingosine kinase 1a, His-tag Recombinant

Catalog #

40613

$415 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His-tag, MW=47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

SphK1, sphingosine kinase 1, SPK1

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

47.1 kDa

Amino Acids

full length

Specific Activity

20 U/µg

Unit Definition

A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.

Genbank #

AF415213

Tag(s)

N-terminal His-tag

References

1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004)
2. Taha, TA et al., FASEB J. 20(3):482-4 (2005)

Storage/Stability

>6 months at –80°C.

Assay Conditions

Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM D-erythrosphingosine, and 0.1-0.5 µg/ml sphingosine kinase 1 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo^® Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Lipids

Regulatory Status

Research Use Only

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Mouse Sphingosine kinase 1a, His-tag Recombinant

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