SPHK1 Assay Kit
The SPHK1 Assay Kit is designed to measure SPHK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Need us to run inhibitor screens or profile your compounds against SPHK1? Check out our Kinase Screening Services.
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 0.5 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
| Catalog Number |
Reagent |
Amount |
Storage |
|
| 40610 | SPHK1, His-tag* | >1 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
| 79686 | ATP (500 μM) | 100 µl | -20°C | |
| Sphingosine (1 mM) | 100 µl | -20°C | ||
| 79696 | 96-well plate, white | 1 | Room Temp |
|
*Excess material has been provided for ease of retrieval from the vial.
SPHK1 is a lipid enzyme that catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP). It has been implicated in tumor progression through cell proliferation and motility. High levels of SPHK1 have been associated with increased mortality in various forms of human cancer.
1. Shida, D., et al. 2008. "Targeting SphK1 as a new strategy against cancer." Current Drug Targets 9(8): 662-673.
2. Xia, J., et al. 2012. "miR‐124 inhibits cell proliferation in gastric cancer through down‐regulation of SPHK1." The Journal of Pathology 227(4): 470-480.
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