Metabolic Enzymes Screening and Profiling Services

Accelerate your drug discovery program with Metabolic Enzyme Screening and Profiling Services from BPS Bioscience, Inc. Trust us to provide you with high quality data with fast turnaround time. Our team of experts along with our broad services portfolio makes it easy to:

  • Screen for inhibitors/targets using our panel of 20+ assays
  • Select from IC50 determination and single point concentrations
  • Receive data within days of compound submission
  • Perform follow-up studies using the same proteins manufactured in-house
  • Get questions answered or project guidance in a time-efficient manner

Biochemical Assays:

ACC2 IDH1(R132H)
ACLY IDH2
ARG1/2 IDH2(R140Q)
CA1 IDO/TDO
CA2 MAT2A
COX1 MTAP
COX2 NAMPT
FUK NMNAT
FDH PKL
GLS1 PKR
GK1 PKM1
IDH1 PKM2
  TPH1 /TPH2 

Background:

Metabolic enzymes carry out a variety of cellular functions necessary for survival and homeostasis, including proteolysis and digestion, cellular respiration, energy storage, transcription, and response to the environment. These life-sustaining pathways are vital for growth and maintenance of cellular integrity. Metabolic enzymes encompass a wide range of different protein classes, including carboxylases, dehydrogenases, lipoxygenases, oxidoreductases, kinases, lyases, transferases, and more.

Disregulation of metabolic pathways has been linked to diabetes, obesity, hypertension, osteoporosis, hormonal disorders, cancer, and many other diseases. Given the importance of these essential pathways to cellular integrity and survival, it is not surprising that disregulation of delicate metabolic pathways has been linked to a number of human diseases. Targeting metabolic enzymes is of high interest in drug development programs for metabolic diseases such as diabetes, obesity and cancer. For example, Acetyl-coenzymes A (ACC1 and ACC2) are metabolic enzymes involved in fatty acid metabolism and is are attractive metabolic targets against diabetes and obesity.Phosphoglycerate Dehydrogenase (PHGDH) serves as a marker for aggressive breast cancer and melanoma.Nicotinamide Phosphoribosyltransferase (PBEF) regulates insulin secretion, making it a target for type 2 diabetes and tumor metastasis. ATP Citrate Lyase is required for histone acetylation and is highly upregulated in many cancer cells. HSPs are an ideal type of metabolic enzymes used for the study and effectiveness of cancer vaccines, the discovery of anticancer therapeutics, and the development of infectious disease treatments. Finally, Isocitrate Dehydrogenases (IDH1 & 2) are prominant markers for leukemia and brain tumors.

BPS’s portfolio of metabolic enzymes currently includes over 50 different enzymes and is continuously expanding.  Also, learn more about the Screening and Profiling Services for Metabolic Enzymes.

References: 
1.    Hsu, P.P., et al., Cell. 2008. 134(5): 703-707. 
2.    Maritim, A.C., et al., J. Biochem. Mol. Toxicol. 2003. 17(1): 24-38.

 

Related Technologies:

  • Chemiluminescent
  • Time-resolved fluorometric

Contact us today for more information about our Screening and Profiling Services. Tailor our services to meet your needs: Learn more about our Custom Services.

Not quite what you’re looking for? Our Custom Assay Development Services allow for flexibility to meet your specific research needs